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parp1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc parp1

    Parp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 15345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/parp/PARP+Antibody/pmc13669479-13-0-7
    Average 98 stars, based on 15345 article reviews
    parp1 - by Bioz Stars, 2026-10
    98/100 stars

    Images

    1) Product Images from "Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21"

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

    Journal: Advanced Science

    doi: 10.1002/advs.78290


    Figure Legend Snippet:

    Techniques Used: Cell-Signaling

    Related Articles

    Bioprocessing:

    Article Title: Apoptosis-inducing potential of tilorone dihydrochloride in triple-negative breast cancer: in silico and in vitro evidences.
    Article Snippet: Tilorone is an antiviral agent with interferon-inducing properties.. The present study focused on evaluating the apoptosisinducing effects of tilorone in triple-negative breast cancer (TNBC) using in silico and in vitro models.. Molecular docking and dynamics were performed to investigate the interactions of cGAMP and tilorone with human STING.

    Cell-Signaling:

    Article Title: Apoptosis-inducing potential of tilorone dihydrochloride in triple-negative breast cancer: in silico and in vitro evidences.
    Article Snippet: Tilorone is an antiviral agent with interferon-inducing properties.. The present study focused on evaluating the apoptosisinducing effects of tilorone in triple-negative breast cancer (TNBC) using in silico and in vitro models.. Molecular docking and dynamics were performed to investigate the interactions of cGAMP and tilorone with human STING.

    Article Title: MerTK inhibition by UNC569 triggers DNA damage and JNK/p38 MAPK cascade-driven apoptosis in pancreatic cancer.
    Article Snippet: .. The following primary antibodies were used: p-Rb (8516), p21 (2947), Snail (3879), cleaved caspase3 (9669), PARP (9542), JNK (9252), p-p38 (4511), γ-H2A.X (9718), p-JNK (4668) and p38 (8690) from Cell Signaling Technology (Danvers, USA); p-MerTK (ab14921) from Abcam (Cambridge, UK); Rb (A3618) and p-CDK6 (AP0326) from ABclonal (Wuhan, China); β-actin (P30002) from Abmart (Shanghai, China); N-cadherin (22018-1-AP) and Ecadherin (20874-1-AP) from Proteintech (Wuhan, China); Cyclin B1 (sc-245) and c-Myc (sc-42) from Santa Cruz Biotechnology (Dallas, USA); CDK6 (VWA00022) and p27 (AHP2662) from BioRad (Hercules, USA). ..

    Article Title: Development of Styryl‐Modified 3,4‐Dihydropyrimidin‐2(1 H )‐ones as Potential Antitumor Agents
    Article Snippet: SDS PAGE were performed using Mini‐PROTEAN TGX Precast Gels (Bio‐Rad) and transferred using Trans‐Blot Turbo Transfer System (Bio‐Rad). .. Primary antibody, PARP (95425, Cell signaling), Caspase 3 (ab32042, Abcam), GAPDH (ab8245, Abcam), p53 (ab32389, Abcam), p21 (ab109199, Abcam), c‐Myc (ab32072, Abcam), β‐actin (ab8227, Abcam), lamin and α‐tubulin (ab7291, Abcam) were blotted for overnight and Goat Anti‐Rabbit HRP (ab205718) and goat antimouse IgG‐HRP (sc2005, Santa Cruz Biotechnology, Inc.) were blotted for secondary antibody 1 h. ImageQuantTMLAS 500 (29‐0050‐63, GE) has been used to detect protein bands. .. Diclofenac, progesterone, nicardipine, erythromycin, chlorambucil, and desipramine HCl were obtained from Sigma (St. Louis, MO, USA).

    other:

    Article Title: Overcoming acquired doxorubicin resistance of ovarian carcinoma cells by verapamil-mediated promotion of DNA damage-driven cytotoxicity
    Article Snippet: The following primary antibodies were used: Copper transporting ATPase (ATP7A), extracellular regulated kinase 2 (ERK2), phosphorylated (p)-histone H3 (Ser10) from Thermo Fisher Scientific Inc., cleaved caspase-7 (Asp198), p-Chk1 (Ser 345), cyclin B1, galactosidase β (E2U2I), GAPDH (14C10), MDR1/ABCB1 (D3H1Q), p-P53 (S15), PARP, TopBP1(D8G4L), topoisomerase IIa (D10G9), 53BP1 and Ki67 were from Cell Signaling Technology Inc., pChk2 (T68) [Y171], copper uptake protein 1 (CTR1/SLC31A1) [EPR7936] and Rad51 from Abcam, γH2AX (Ser 139) clone JBW301, p-KAP-1 (S824) and p-RPA32 (S4/S8) from Bethyl Laboratories Inc., organic cation transporter-2 (OCT2) from Biozol Diagnostics Vertrieb GmbH, p16 (F-12) and p21 (C-19) from Santa Cruz Biotechnology, Inc. As secondary antibodies, horseradish peroxidase-conjugated secondary antibodies goat anti-mouse IgG and mouse anti-rabbit IgG were used (Rockland Immunochemicals Inc.).

    Article Title: Development of Styryl-Modified 3,4-Dihydropyrimidin-2(1H)-ones as Potential Antitumor Agents.
    Article Snippet: 20 μg lysate was prepared with 2x Laemmli Sample Buffer (1610737, Biorad).

    Electrophoresis:

    Article Title: The ribonuclease ZC3H12A stabilizes BRCA1 mRNA in DNA damage response.
    Article Snippet: .. Samples were then loaded onto 4%-12% Tris polyacrylamide gels and resolved by electrophoresis at 150 V for 1-1.5 h. The proteins were transferred onto nitrocellulose (NC) membranes at 400 mA for 1-3 h. Membranes were blocked with 5% non-fat milk for 1 h at room temperature and subsequently incubated with specific primary antibodies against ZC3H12A (GeneTex, 1:4000), BRCA1 (Proteintech, 1:2000), γH2AX (Sigma, 1:2000), PARP (CST, 1:1000), MRE11 (Novus, 1:4000), XRCC1 (Proteintech, 1:2000), Ku70 (ABclonal, 1:2000), Ku80 (ABclonal, 1:2000), β-tubulin (Proteintech, 1:10000), TBK1 (Immunoway, 1:2000) and IKKε (Immunoway, 1:2000). .. HRP-conjugated anti-Flag (Abcam, 1:4000), HRP-conjugated anti-Lamin b1 (Proteintech, 1:10000), HRP-conjugated anti-GAPDH (Proteintech,1:10000) were incubated overnight at 4◦C or for 1 h at room temperature.

    Incubation:

    Article Title: The ribonuclease ZC3H12A stabilizes BRCA1 mRNA in DNA damage response.
    Article Snippet: .. Samples were then loaded onto 4%-12% Tris polyacrylamide gels and resolved by electrophoresis at 150 V for 1-1.5 h. The proteins were transferred onto nitrocellulose (NC) membranes at 400 mA for 1-3 h. Membranes were blocked with 5% non-fat milk for 1 h at room temperature and subsequently incubated with specific primary antibodies against ZC3H12A (GeneTex, 1:4000), BRCA1 (Proteintech, 1:2000), γH2AX (Sigma, 1:2000), PARP (CST, 1:1000), MRE11 (Novus, 1:4000), XRCC1 (Proteintech, 1:2000), Ku70 (ABclonal, 1:2000), Ku80 (ABclonal, 1:2000), β-tubulin (Proteintech, 1:10000), TBK1 (Immunoway, 1:2000) and IKKε (Immunoway, 1:2000). .. HRP-conjugated anti-Flag (Abcam, 1:4000), HRP-conjugated anti-Lamin b1 (Proteintech, 1:10000), HRP-conjugated anti-GAPDH (Proteintech,1:10000) were incubated overnight at 4◦C or for 1 h at room temperature.



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    Image Search Results


    Journal: iScience

    Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

    doi: 10.1016/j.isci.2026.117627

    Figure Lengend Snippet:

    Article Snippet: Anti-PARP1 , Proteintech Group , Cat# 13371-1-AP; RRID: AB_2160459.

    Techniques: Cell-Signaling, Recombinant, Transfection, Caspase-3 Activity Assay, Protein Extraction, Knock-Out, Sequencing, Control, Plasmid Preparation, Software, Analysis

    Journal: Advanced Science

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

    doi: 10.1002/advs.78290

    Figure Lengend Snippet:

    Article Snippet: PARP1 , 9542S , Cell Signaling Technology (CST) , AB_2160739.

    Techniques: Cell-Signaling